3D Cell Culture Stimulates the Secretion of in vivo Like Extracellular Vesicles

September 9, 2019

Recently researchers at The University of Kansas (Lawrence, Kansas) demonstrated 3D cell spheroids culture stimulates the secretion of in vivo like extracellular vesicles (EVs or exosomes). The EVs secreted by cancer cell spheroids cultured in 3D PGmatrix hydrogel scaffolding showed about 96% similarity to in vivo circulating EVs derived from cancer patient plasma according to RNA sequencing analysis. While the EVs from the same cells cultured using traditional flat surface (2D) method are not relevant.  

“This work also suggests that tackling EV molecular alterations secreted into interstitial fluids can provide an alternative, non-invasive approach for investigating 3D tissue behaviors at the molecular precision” said Dr. M. He, PI and Assistant Professor from The University of Kansas with expertise in 3D bioprinting and cellular engineering.  She also suggests that this 3D model would allow scientists obtain accurate tumor biomarker information and better understanding tumor progression and metastasis. 

PepGel platform technologies provide advanced peptide hydrogels for life science and biomedical applications. The PGmatrix kit is one of our popular products for robust 3D cells/spheroids/organoids cultures. Most importantly, spheroids are biologically formed by cell themselves in PGmatrix hydrogel scaffold.  

For more detailed information about this work, please click the link for full article at www.nature.com/articles/s41598-019-49671-3. For PGmatrix kit information, please visit www.pepgel.com, or send a note to info@pepgel.com. 

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